| ACTB

Actin beta (HUGO Gene Nomenclature Committee abbreviation ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	100	50	25	12.5	6.25	3.12	1.56	0
OD	1.94	1.32	0.78	0.51	0.3	0.18	0.12	0.08
Corrected OD	1.86	1.24	0.7	0.43	0.22	0.1	0.04	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse ACTB spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Cell Culture Supernates(n=8)	90-105	100

 

| Linearity

Samples were spiked with high concentrations of Mouse ACTB and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Cell Culture Supernates(n=4)
1 : 2	Range (%)	88-111
	Average (%)	97
1 : 4	Range (%)	103-118
	Average (%)	108